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pET28a Plasmid

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin …

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Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complex.

Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complex.

Non-destructive analysis of cells on the molecular degree is of vital significance for cell analysis. At current, immunoassay-based and aptamer-based strategies can obtain non-structural damaging cell analysis, however nonetheless result in modifications in cells on the molecular degree. Here, we’ve proposed a dual-terminal amplification (DTA) technique, which permits nondestructive analysis of membrane protein MUC1 with …

Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complex. Read More »

DNA Barcoding in Nonhuman Primates Reveals Important Limitations in Retrovirus Integration Site Analysis.

DNA Barcoding in Nonhuman Primates Reveals Important Limitations in Retrovirus Integration Site Analysis.

In vivo monitoring of retrovirus-tagged blood stem and progenitor cells is used to check hematopoiesis. Two strategies are used most incessantly: sequencing the locus of retrovirus insertion, termed integration web site evaluation, or retrovirus DNA barcode sequencing. Of these, integration web site evaluation is at present the one out there method for monitoring clonal swimming pools …

DNA Barcoding in Nonhuman Primates Reveals Important Limitations in Retrovirus Integration Site Analysis. Read More »

Combined DNA Index System (CODIS)-Based analysis of untested sexual assault evidence in Palm Beach County Florida.

Combined DNA Index System (CODIS)-Based analysis of untested sexual assault evidence in Palm Beach County Florida.

In August 2015, the Palm Beach County Sheriff’s Office (PBSO) initiated a CODIS-Based Sexual Assault Evidence Testing Initiative, hereto known as the Initiative, as a direct outcome of the continued nationwide inquiry in regards to the quantity of untested sexual assault circumstances having DNA evidence saved in regulation enforcement company vaults. The Initiative was designed to analysis sexual …

Combined DNA Index System (CODIS)-Based analysis of untested sexual assault evidence in Palm Beach County Florida. Read More »

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