Analysis of Epigenetic Alterations in Homologous Recombination DNA Repair Genes in Male Breast Cancer.

BACKGROUNDMale breast most cancers (BC) is a definite neoplasm with low however rising incidence, incessantly identified as superior stage illness. Considering the relevance of altered homologous recombination restore (HRR) in male BC, we aimed to discover the biomarker potential of aberrant promoter methylation of ATM, BRCA1, PALB2, RAD51B, and XRCC3.Formalin-fixed paraffin-embedded (FFPE) tissue samples from 128 male BC sufferers, paired adjoining regular tissue and 19 gynecomastia instances have been collected and assessed by quantitative methylation-specific PCR (qMSP). Non-parametric exams have been used to match methylation ranges between tumor and non-tumor samples and to hunt for associations with clinicopathological variables.

RESULTSOnly RAD51B and XRCC3 disclosed important variations between tumor and gynecomastia (p < 0.0001 and p = 0.020, respectively). Assembled in a panel, RAD51B and XRCC3 promoter methylation discriminated male BC from gynecomastia with 91.5% sensitivity, 89.5% specificity, and 91.2% accuracy.

Mouse Nuclear Factor Kappa B (NFkB) ELISA Kit
DLR-NFkB-Mu-48T	DL Develop	48T	EUR 540
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Nuclear Factor Kappa B (NFkB) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Nuclear Factor Kappa B (NFkB) ELISA Kit
DLR-NFkB-Mu-96T	DL Develop	96T	EUR 698.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Nuclear Factor Kappa B (NFkB) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Nuclear Factor Kappa B (NFkB) ELISA Kit
DLR-NFkB-Ra-48T	DL Develop	48T	EUR 633.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Nuclear Factor Kappa B (NFkB) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Nuclear Factor Kappa B (NFkB) ELISA Kit
DLR-NFkB-Ra-96T	DL Develop	96T	EUR 828
Description: A sandwich quantitative ELISA assay kit for detection of Rat Nuclear Factor Kappa B (NFkB) in samples from tissue homogenates, cell lysates or other biological fluids.

Chicken Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Ch-48Tests	Reddot Biotech	48 Tests	EUR 640.8

Chicken Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Ch-96Tests	Reddot Biotech	96 Tests	EUR 890.4

Human Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Hu-48Tests	Reddot Biotech	48 Tests	EUR 600

Human Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Hu-96Tests	Reddot Biotech	96 Tests	EUR 830.4

Mouse Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Mu-48Tests	Reddot Biotech	48 Tests	EUR 535.2

Mouse Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Mu-96Tests	Reddot Biotech	96 Tests	EUR 738

Rat Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Ra-48Tests	Reddot Biotech	48 Tests	EUR 640.8

Rat Nuclear Factor Kappa B (NFkB) ELISA Kit
RD-NFkB-Ra-96Tests	Reddot Biotech	96 Tests	EUR 890.4

Chicken Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Ch-48Tests	Reddot Biotech	48 Tests	EUR 669.6

Chicken Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Ch-96Tests	Reddot Biotech	96 Tests	EUR 931.2

Human Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Hu-48Tests	Reddot Biotech	48 Tests	EUR 626.4

Human Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Hu-96Tests	Reddot Biotech	96 Tests	EUR 868.8

Mouse Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Mu-48Tests	Reddot Biotech	48 Tests	EUR 558

Mouse Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Mu-96Tests	Reddot Biotech	96 Tests	EUR 771.6

Rat Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Ra-48Tests	Reddot Biotech	48 Tests	EUR 669.6

Rat Nuclear Factor Kappa B (NFkB) ELISA Kit
RDR-NFkB-Ra-96Tests	Reddot Biotech	96 Tests	EUR 931.2

IKB
EF017836	Lifescience Market	96 Tests	EUR 826.8

IkB alpha antibody
70R-11718	Fitzgerald	100 ug	EUR 483.6
Description: Rabbit polyclonal IkB alpha antibody

IkB alpha antibody
10R-8517	Fitzgerald	100 ul	EUR 471.6
Description: Mouse monoclonal IkB alpha antibody

IkB alpha antibody
70R-37560	Fitzgerald	100 ug	EUR 327.6
Description: Rabbit Polyclonal IkB alpha antibody

IKB alpha antibody
70R-50122	Fitzgerald	100 ul	EUR 344.4
Description: Purified Polyclonal IKB alpha antibody

IKB alpha antibody
70R-50123	Fitzgerald	100 ul	EUR 292.8
Description: Purified Polyclonal IKB alpha antibody

IKB alpha antibody
70R-50124	Fitzgerald	100 ul	EUR 292.8
Description: Purified Polyclonal IKB alpha antibody

IKB alpha antibody
70R-34201	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal IKB alpha antibody

IKB alpha antibody
70R-34217	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal IKB alpha antibody

IKB alpha antibody
70R-35813	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal IKB alpha antibody

IKB alpha antibody
70R-13374	Fitzgerald	100 ul	EUR 548.4
Description: Affinity purified Rabbit polyclonal IKB alpha antibody

IKB alpha Antibody
48695-100ul	SAB	100ul	EUR 399.6

IKB alpha Antibody
48695-50ul	SAB	50ul	EUR 286.8

IKB beta Antibody
49019-100ul	SAB	100ul	EUR 399.6

IKB beta Antibody
49019-50ul	SAB	50ul	EUR 286.8

IKB epsilon Antibody
49630-100ul	SAB	100ul	EUR 399.6

IKB epsilon Antibody
49630-50ul	SAB	50ul	EUR 286.8

IkB alpha antibody
10R-6740	Fitzgerald	100 ug	EUR 846
Description: Mouse monoclonal IkB alpha antibody

IkB alpha antibody
20R-1504	Fitzgerald	100 ug	EUR 807.6
Description: Rabbit polyclonal IkB alpha antibody

IKB alpha antibody
20R-2004	Fitzgerald	50 ug	EUR 337.2
Description: Rabbit polyclonal IKB alpha antibody

IKB alpha antibody
20R-2012	Fitzgerald	50 ug	EUR 337.2
Description: Rabbit polyclonal IKB alpha antibody

IkB Epsilon antibody
20R-2055	Fitzgerald	50 ug	EUR 337.2
Description: Rabbit polyclonal IkB Epsilon antibody

IKB beta antibody
20R-2126	Fitzgerald	50 ug	EUR 337.2
Description: Rabbit polyclonal IKB beta antibody

IKB alpha antibody
20R-2298	Fitzgerald	50 ug	EUR 337.2
Description: Rabbit polyclonal IKB alpha antibody

IKB alpha antibody
20R-2343	Fitzgerald	50 ug	EUR 337.2
Description: Rabbit polyclonal IKB alpha antibody

IkB Epsilon antibody
20R-2449	Fitzgerald	50 ug	EUR 337.2
Description: Rabbit polyclonal IkB Epsilon antibody

IkB-alpha Antibody
29054-100ul	SAB	100ul	EUR 302.4

IkB-beta Antibody
29055-100ul	SAB	100ul	EUR 302.4

IKB alpha Antibody
AF7776	Affbiotech	200ul	EUR 540

IKB alpha Antibody
AF5002	Affbiotech	200ul	EUR 420

IKB epsilon Antibody
AF6002	Affbiotech	200ul	EUR 420

IKB alpha Antibody
AF6239	Affbiotech	200ul	EUR 420

IKB beta Antibody
AF6447	Affbiotech	200ul	EUR 420

IKB beta Antibody
AF6448	Affbiotech	200ul	EUR 420

IKB a protein
E20-80050	EnoGene	20ug	EUR 489.6

IKB a protein
E20-80051	EnoGene	20ug	EUR 489.6

IKB b protein
E20-80052	EnoGene	20ug	EUR 489.6

IKB b protein
E20-80053	EnoGene	20ug	EUR 489.6

IkB ? Polyclonal Antibody
EA339-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against IkB ? from Human/ Mouse/ Rat. This antibody is tested and validated for WB, ELISA, IHC

IkB ? Polyclonal Antibody
EA339-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against IkB ? from Human/ Mouse/ Rat. This antibody is tested and validated for WB, ELISA, IHC

anti-IKB epsilon
YF-PA24239	Abfrontier	50 ul	EUR 400.8
Description: Mouse polyclonal to IKB epsilon

anti-IkB beta
YF-PA13438	Abfrontier	50 ul	EUR 435.6
Description: Mouse polyclonal to IkB beta

anti-IkB beta
YF-PA13439	Abfrontier	100 ug	EUR 483.6
Description: Rabbit polyclonal to IkB beta

IkB beta Antibody
R31705	NSJ Bioreagents	100 ug	EUR 419

IkB alpha Antibody
F50935-0.08ML	NSJ Bioreagents	0.08 ml	EUR 165

IkB alpha Antibody
F50935-0.4ML	NSJ Bioreagents	0.4 ml	EUR 379

IKB alpha Rabbit pAb
A1187-100ul	Abclonal	100 ul	EUR 369.6

IKB alpha Rabbit pAb
A1187-200ul	Abclonal	200 ul	EUR 550.8

IKB alpha Rabbit pAb
A1187-20ul	Abclonal	20 ul	EUR 219.6

IKB alpha Rabbit pAb
A1187-50ul	Abclonal	50 ul	EUR 267.6

IKB alpha Rabbit pAb
A11165-100ul	Abclonal	100 ul	EUR 369.6

IKB alpha Rabbit pAb
A11165-200ul	Abclonal	200 ul	EUR 550.8

IKB alpha Rabbit pAb
A11165-20ul	Abclonal	20 ul	EUR 219.6

IKB alpha Rabbit pAb
A11165-50ul	Abclonal	50 ul	EUR 267.6

IKB alpha Rabbit pAb
A11168-100ul	Abclonal	100 ul	EUR 369.6

IKB alpha Rabbit pAb
A11168-200ul	Abclonal	200 ul	EUR 550.8

IKB alpha Rabbit pAb
A11168-20ul	Abclonal	20 ul	EUR 219.6

IKB alpha Rabbit pAb
A11168-50ul	Abclonal	50 ul	EUR 267.6

IKB alpha Rabbit pAb
A11474-100ul	Abclonal	100 ul	EUR 369.6

IKB alpha Rabbit pAb
A11474-200ul	Abclonal	200 ul	EUR 550.8

IKB alpha Rabbit pAb
A11474-20ul	Abclonal	20 ul	EUR 219.6

IKB alpha Rabbit pAb
A11474-50ul	Abclonal	50 ul	EUR 267.6

IKB alpha antibody (Tyr42)
70R-37239	Fitzgerald	100 ug	EUR 418.8
Description: Rabbit Polyclonal IKB alpha antibody (Tyr42)

IkB epsilon antibody (Ser22)
70R-37280	Fitzgerald	100 ug	EUR 418.8
Description: Rabbit Polyclonal IkB epsilon antibody (Ser22)

IkB beta antibody (Ser23)
70R-37354	Fitzgerald	100 ug	EUR 418.8
Description: Rabbit Polyclonal IkB beta antibody (Ser23)

IKB alpha antibody (Tyr42)
70R-34200	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal IKB alpha antibody (Tyr42)

IkB alpha antibody (Tyr305)
70R-35607	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal IkB alpha antibody (Tyr305)

Human IkB-delta Antibody
32025-05111	AssayPro	150 ug	EUR 313.2

Biotin-IkB Inhibitor, amide
5-00792	CHI Scientific	4 x 1mg	Ask for price

IKB alpha Rabbit pAb
A2457-100ul	Abclonal	100 ul	EUR 460.8

IKB alpha Rabbit pAb
A2457-200ul	Abclonal	200 ul	Ask for price

IKB alpha Rabbit pAb
A2457-20ul	Abclonal	20 ul	Ask for price

IKB alpha Rabbit pAb
A2457-50ul	Abclonal	50 ul	EUR 318

IKB alpha (pY42) Antibody
20-abx141035	Abbexa	EUR 460.80 EUR 727.20 EUR 276.00	100 ul 200 ul 30 ul

IKB Alpha (IKBA) Antibody
20-abx141104	Abbexa	EUR 427.20 EUR 644.40 EUR 260.40	100 ul 200 ul 30 ul

IKB Epsilon (IKBE) Antibody
20-abx133020	Abbexa	EUR 360.00 EUR 526.80 EUR 226.80	100 ul 200 ul 30 ul

IKB Alpha (IKBA) Antibody
20-abx134360	Abbexa	EUR 427.20 EUR 644.40 EUR 260.40	100 ul 200 ul 30 ul

IKB epsilon (pS22) Antibody
20-abx134997	Abbexa	EUR 460.80 EUR 727.20 EUR 276.00	100 ul 200 ul 30 ul

IKB epsilon Blocking Peptide
20-abx161691	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

IKB alpha Blocking Peptide
20-abx061802	Abbexa	EUR 309.60 EUR 460.80	1 mg 5 mg

IKB alpha Blocking Peptide
20-abx062998	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

IKB alpha Blocking Peptide
20-abx062999	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

IKB Alpha (IKBA) Antibody
20-abx008935	Abbexa	EUR 360.00 EUR 526.80 EUR 226.80	100 ul 200 ul 30 ul

IKB Alpha (IKBA) Antibody
20-abx008936	Abbexa	EUR 360.00 EUR 526.80 EUR 226.80	100 ul 200 ul 30 ul

IkB beta (pT19) Antibody
abx011060-100ug	Abbexa	100 ug	EUR 526.8

IkB beta (pS23) Antibody
abx011062-100ug	Abbexa	100 ug	EUR 526.8

IkB epsilon (pS22) Antibody
abx011063-100ug	Abbexa	100 ug	EUR 526.8

IkB alpha (pY305) Antibody
abx011261-100ug	Abbexa	100 ug	EUR 526.8

IkB Alpha (IKBA) Antibody
abx031605-400ul	Abbexa	400 ul	EUR 627.6

IkB Alpha (IKBA) Antibody
abx031605-80l	Abbexa	80 µl	EUR 343.2

IKB alpha Rabbit mAb
A19714-100ul	Abclonal	100 ul	EUR 492

IKB alpha Rabbit mAb
A19714-200ul	Abclonal	200 ul	EUR 685.2

IKB alpha Rabbit mAb
A19714-20ul	Abclonal	20 ul	EUR 265.2

IKB alpha Rabbit mAb
A19714-50ul	Abclonal	50 ul	EUR 344.4

IkB Alpha (IKBA) Antibody
abx234196-100ug	Abbexa	100 ug	EUR 577.2

IkB Alpha (IKBA) Antibody
abx234197-100ug	Abbexa	100 ug	EUR 577.2

IkB Alpha (IKBA) Antibody
abx234198-100ug	Abbexa	100 ug	EUR 610.8

IkB Beta (IKBB) Antibody
abx234199-100ug	Abbexa	100 ug	EUR 577.2

IKB alpha Conjugated Antibody
C48695	SAB	100ul	EUR 476.4

IKB beta Conjugated Antibody
C49019	SAB	100ul	EUR 476.4

IKB epsilon Conjugated Antibody
C49630	SAB	100ul	EUR 476.4

IkB-alpha Conjugated Antibody
C29054	SAB	100ul	EUR 476.4

IkB-beta Conjugated Antibody
C29055	SAB	100ul	EUR 476.4

Porcine IKB ELISA Kit
EPI0032	Abclonal	96Tests	EUR 625.2

Rabbit IKB ELISA Kit
ERTI0032	Abclonal	96Tests	EUR 625.2

Rat IKB ELISA Kit
ERI0032	Abclonal	96Tests	EUR 625.2

Mouse IKB ELISA Kit
EMI0032	Abclonal	96Tests	EUR 625.2

Monkey IKB ELISA Kit
EMKI0032	Abclonal	96Tests	EUR 625.2

Anti-IkB alpha antibody
PAab04196	Lifescience Market	100 ug	EUR 426

Anti-IkB alpha antibody
PAab04197	Lifescience Market	100 ug	EUR 426

Anti-IkB alpha antibody
PAab04198	Lifescience Market	100 ug	EUR 463.2

Anti-IkB beta antibody
PAab04199	Lifescience Market	100 ug	EUR 426

Human IKB ELISA Kit
EHI0032	Abclonal	96Tests	EUR 625.2

Goat IKB ELISA Kit
EGTI0032	Abclonal	96Tests	EUR 625.2

Bovine IKB ELISA Kit
EBI0032	Abclonal	96Tests	EUR 625.2

Canine IKB ELISA Kit
ECI0032	Abclonal	96Tests	EUR 625.2

Chicken IKB ELISA Kit
ECKI0032	Abclonal	96Tests	EUR 625.2

IkB ? Rabbit Polyclonal Antibody
ES8561-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against IkB ? Rabbit from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC

IkB ? Rabbit Polyclonal Antibody
ES8561-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against IkB ? Rabbit from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC

anti- IkB alpha antibody
FNab04196	FN Test	100µg	EUR 606.3
Description: Antibody raised against IkB alpha

anti- IkB alpha antibody
FNab04197	FN Test	100µg	EUR 606.3
Description: Antibody raised against IkB alpha

anti- IkB alpha antibody
FNab04198	FN Test	100µg	EUR 658.5
Description: Antibody raised against IkB alpha

anti- IkB beta antibody
FNab04199	FN Test	100µg	EUR 606.3
Description: Antibody raised against IkB beta

Sheep IKB ELISA Kit
ESI0032	Abclonal	96Tests	EUR 625.2

Anti-IkB alpha antibody
STJ98869	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to IkB alpha.

Anti-IkB-alpha antibody
STJ99105	St John's Laboratory	200 µl	EUR 236.4
Description: Mouse monoclonal to IkB-alpha.

Anti-IkB beta (2B11)
YF-MA14441	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to IkB beta

Anti-IkB beta (3E11)
YF-MA14442	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to IkB beta

Anti-IkB beta (3B5)
YF-MA14443	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to IkB beta

Anti-IkB beta (1G6)
YF-MA14444	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to IkB beta

Anti-IkB beta (1B5)
YF-MA10628	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to IkB beta

IKB alpha Polyclonal Antibody
A73253-050	EpiGentek	50 ul	EUR 377.3

IKB alpha Polyclonal Antibody
A73253-100	EpiGentek	100 ul	EUR 577.5

IKB alpha Polyclonal Antibody
A73253	EpiGentek	EUR 377.30 EUR 577.50	50 ul 100 ul

IKB epsilon Antibody / NFKBIE
RQ4686	NSJ Bioreagents	100ul	EUR 419

NFKBIE Antibody (IkB epsilon)
F53208-0.08ML	NSJ Bioreagents	0.08 ml	EUR 165

NFKBIE Antibody (IkB epsilon)
F53341-0.08ML	NSJ Bioreagents	0.08 ml	EUR 165

IKB alpha Antibody (IKBa)
F48068-0.08ML	NSJ Bioreagents	0.08 ml	EUR 165

Anti-IkB alpha Antibody
A1763-100	Biovision	each	EUR 574.8

Recombinant purified human IKB-beta (E., coli; GST-tag; IKB-beta)
IKBB25-R-10	Alpha Diagnostics	10 ug	EUR 343.2

NFkB p65 antibody
70R-11575	Fitzgerald	100 ug	EUR 483.6
Description: Rabbit polyclonal NFkB p65 antibody

NFkB p50 antibody
70R-11768	Fitzgerald	100 ug	EUR 483.6
Description: Rabbit polyclonal NFkB p50 antibody

NFkB p105 antibody
70R-12174	Fitzgerald	200 ug	EUR 483.6
Description: Rabbit polyclonal NFkB p105 antibody

NFkB p52 antibody
10R-8543	Fitzgerald	100 ul	EUR 471.6
Description: Mouse monoclonal NFkB p52 antibody

NFkB p65 antibody
70R-37450	Fitzgerald	100 ug	EUR 327.6
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody
70R-37451	Fitzgerald	100 ug	EUR 327.6
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody
70R-37452	Fitzgerald	100 ug	EUR 327.6
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody
70R-37453	Fitzgerald	100 ug	EUR 327.6
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p65 antibody
70R-37454	Fitzgerald	100 ug	EUR 327.6
Description: Rabbit Polyclonal NFkB p65 antibody

NFkB p100 antibody
70R-35337	Fitzgerald	100 ug	EUR 392.4
Description: Purified Rabbit polyclonal NFkB p100 antibody

NFKB p105 antibody
70R-35682	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal NFKB p105 antibody

NFkB p65 antibody
70R-36057	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal NFkB p65 antibody

Moreover, promoter methylation ranges have been decrease in paired non-tumor tissues, evaluating to tumor samples. No associations have been discovered between epigenetic alterations and clinicopathological options, in addition to with RAD51 and XRCC3 immunoexpression and methylation ranges.

CONCLUSIONQuantitative promoter methylation of RAD51B and XRCC3 constitutes a promising and correct biomarker for male BC. Validation in bigger sequence and in liquid biopsies is warranted to verify its usefulness in detection and monitoring settings.