Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complex.

Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complex.

Non-destructive analysis of cells on the molecular degree is of vital significance for cell analysis. At current, immunoassay-based and aptamer-based strategies can obtain non-structural damaging cell analysis, however nonetheless result in modifications in cells on the molecular degree. Here, we’ve proposed a dual-terminal amplification (DTA) technique, which permits nondestructive analysis of membrane protein MUC1 with out the impact on protein expression and cell viability in living cells. 

Methods: A fluorophore (Cy5)-labeled DNA ternary complicated consisting of three oligonucleotides is designed. It can acknowledge MUC1 via its aptamer area, and thus make the MUC1 of cells seen beneath a fluorescence microscope.

When DNA polymerase is added, dual-terminal amplification is carried out. One path dissociates aptamer from MUC1, and the opposite path, also called rolling circle amplification (RCA), produces lengthy linear DNA strands, which could be additional adopted for quantitative analysis of MUC1. In this manner, all reagents are faraway from the floor of the cells after the analysis, which permits nondestructive analysis.

Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complex.
Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complicated.

NFκB Pathway Sampler Kit

E051021 5x50ug
EUR 475
Description: Available in various conjugation types.

Wnt pathway activator 1

T17256-10mg 10mg Ask for price
Description: Wnt pathway activator 1

Wnt pathway activator 1

T17256-1g 1g Ask for price
Description: Wnt pathway activator 1

Wnt pathway activator 1

T17256-1mg 1mg Ask for price
Description: Wnt pathway activator 1

Wnt pathway activator 1

T17256-50mg 50mg Ask for price
Description: Wnt pathway activator 1

Wnt pathway activator 1

T17256-5mg 5mg Ask for price
Description: Wnt pathway activator 1

Wnt pathway activator 2

T13345-10mg 10mg Ask for price
Description: Wnt pathway activator 2

Wnt pathway activator 2

T13345-1g 1g Ask for price
Description: Wnt pathway activator 2

Wnt pathway activator 2

T13345-1mg 1mg Ask for price
Description: Wnt pathway activator 2

Wnt pathway activator 2

T13345-50mg 50mg Ask for price
Description: Wnt pathway activator 2

Wnt pathway activator 2

T13345-5mg 5mg Ask for price
Description: Wnt pathway activator 2

Integrin Pathway Sampler Kit

E051025 5x50ug
EUR 475
Description: Available in various conjugation types.

Human Notch Signaling Pathway

HNOT-I 1 set
EUR 657.6

Hh Signaling Pathway Antagonist

1659-1 each
EUR 216

Tissue factor pathway inhibitor

AP79938 1mg
EUR 2640

Tissue factor pathway inhibitor

AP88123 1mg
EUR 2640

Tissue factor pathway inhibitor

AP89055 1mg
EUR 2640

AP1 Reporter Kit (JNK Pathway)

60612 500 rxns.
EUR 565
Description: The AP1 Reporter Kit is designed for monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured cells. The kit contains a transfection-ready AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.

JAK/STAT Pathway Sampler Kit

E051015 5x50ug
EUR 475
Description: Available in various conjugation types.

AP1 Reporter Kit (JNK Pathway)

GWB-PSB7DB 500reactions Ask for price

Human Calcium Regulation Pathway

HCAR-I 1 set
EUR 657.6

β -Catenin Pathway Sampler Kit

E051023 5x50ug
EUR 475
Description: Available in various conjugation types.

Phospho-AKT Pathway Sampler Kit

E051002 5x50ug
EUR 700
Description: Available in various conjugation types.

Phospho-PLC Pathway Sampler Kit

E051020 5x50ug
EUR 700
Description: Available in various conjugation types.

Notch1 Pathway Reporter Kit (Human)

79503 500 rxns.
EUR 650
Description: Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Human NOTCH1 that has a deletion of the entire extracellular domain, leaving the transmembrane and intracellular domains intact (Notch1DE). Inside the cells, the NOTCH1 DE is cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Human Notch1DE. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Mouse Notch1 NICD expression vector, which is constitutively active without processing by γ-secretase (BPS Bioscience #79504)._x000D_

Phospho-MAPK Pathway Sampler Kit

E051018 5x50ug
EUR 700
Description: Available in various conjugation types.

Phospho-NFκB Pathway Sampler Kit

E051022 5x50ug
EUR 700
Description: Available in various conjugation types.

Tissue factor pathway inhibitor 2

AP79755 1mg
EUR 2640

Tissue factor pathway inhibitor 2

AP88049 1mg
EUR 2640

Tissue factor pathway inhibitor 2

AP88141 1mg
EUR 2640

Human Cancer Pathway Primer Library

HCAN-I 1 set
EUR 657.6

TOR Signaling Pathway Detection Set

PSI-1805 1 Set
EUR 1287.6
Description: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GΒL, which stimulates TOR’s kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.;;For images please see PDF data sheet

Human Hippo Signaling Primer Pathway

HHPO-I 1 set
EUR 657.6

FOXO Reporter Kit (PI3K/AKT Pathway)

60643 500 rxns.
EUR 565
Description: The FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for the transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector also contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

JAK pathway ISRE reporter cell line

GWB-PSC83D 1.5x10(6)cells Ask for price

Phospho-Integrin Pathway Sampler Kit

E051026 5x50ug
EUR 700
Description: Available in various conjugation types.

Tissue Factor Pathway Inhibitor Antibody

GWB-626C0B 0.1 mg Ask for price

Myc Reporter Kit (Myc Signaling Pathway)

60519 500 rxns.
EUR 625
Description: The Myc Pathway Reporter kit is designed for monitoring the activity of the Myc signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for c- Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.

Phospho-JAK/STAT Pathway Sampler Kit

E051016 5x50ug
EUR 700
Description: Available in various conjugation types.

Notch1 NICD Pathway Reporter Kit (Mouse)

79504 500 rxns.
EUR 590
Description: Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Mouse NOTCH1 that has a deletion of the entire extracellular domain and transmembrane region (NOTCH1 NICD). Inside the cells, the NOTCH1 NICD is constitutively localized into the nucleus without needing to be cleaved by γ-secretase. The kit also contains CSL (CBF1/RBP-Jκ) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jκ) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Mouse Notch1 NICD, which does not require γ-secretase processing to become active. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Human Notch1DE expression vector (BPS Bioscience #79503), which are dependent on γ-secretase activity to become active.

ARE Reporter Kit (Nrf2 Antioxidant Pathway )

60514 500 rxns.
EUR 650
Description: The ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, which is an Nrf2 pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ARE responsive elements located upstream of a minimal promoter. The ARE reporter is premixed with a constitutively expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. This negative control is critical to determining pathway-specific effects and background luciferase activity.

Phospho-β-Catenin Pathway Sampler Kit

E051024 5x50ug
EUR 700
Description: Available in various conjugation types.

Tissue Factor Pathway Inhibitor Rabbit mAb

E2R27344 100ul
EUR 255
Description: Available in various conjugation types.

Tissue Factor Pathway Inhibitor Rabbit mAb

E2R27346 100ul
EUR 255
Description: Available in various conjugation types.

Tissue Factor Pathway Inhibitor 2 Protein

20-abx263563
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Human Tissue factor pathway inhibitor (TFPI)

1-CSB-EP023437HU
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Description: Recombinant Human Tissue factor pathway inhibitor(TFPI),partial expressed in E.coli

STAT3 Reporter Kit (STAT3 Signaling Pathway)

79730 500 rxns.
EUR 640
Description: The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_
The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

G-protein pathway suppressor 2 Antibody

20-abx137433
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  • 100 ug
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  • 5 ug

Tissue Factor Pathway Inhibitor 2 Protein

abx263563-10mg 10 mg
EUR 1600

Tissue Factor Pathway Inhibitor 2 Protein

abx263563-1mg 1 mg
EUR 225

Tissue Factor Pathway Inhibitor 2 Protein

abx263563-5mg 5 mg
EUR 325

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-YP023437RB
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  • 100ug
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Yeast

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-EP023437RB
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  • 100ug
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in E.coli

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-MP023437RB
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  • 100ug
  • 1MG
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Mammalian cell

Active Tissue Factor Pathway Inhibitor (TFPI)

APA394Hu61 10ug
EUR 360

Rabbit Tissue factor pathway inhibitor (TFPI)

1-CSB-BP023437RB
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  • 100ug
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Baculovirus

SRE Reporter - HEK293 Cell Line (ERK Pathway)

60406 2 vials
EUR 2505
Description: The SRE Reporter - HEK293 cell line is designed for monitoring the activity of the JAK/STAT signaling pathway. The SRE Reporter - HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.

Notch Reporter Assay Kit (cAMP/PKA Pathway)

GWB-PS79B7 500reactions Ask for price

SRE Reporter - HEK293 Cell Line (ERK Pathway)

GWB-PSFA8A 1.5x10^6cells Ask for price

Tissue Factor Pathway Inhibitor (TFPI) Protein

20-abx261170
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  • 1 mg
  • 20 ug
  • 5 ug

Tissue Factor Pathway Inhibitor (TFPI) Protein

abx261170-10g 10 µg
EUR 325

Tissue Factor Pathway Inhibitor (TFPI) Protein

abx261170-2g 2 µg
EUR 225

TCF/LEF Reporter Kit (Wnt Signaling Pathway)

60500 500 rxns.
EUR 655
Description: The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. _x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity. _x000D_

Hippo Pathway TEAD Report Cell Line-MCF7

ABC-RC116D 1 vial Ask for price
Description: The TEAD Reporter andndash; MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line, MCF7. Inside the cells, basal unphosphorylated YAP/TAZ remains in the nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter by the activators of the Hippo pathway.

Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx323593
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  • 100 ug
  • 50 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx433382-200ul 200 ul
EUR 460.8

Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx129517
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  • 100 ug
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  • 1 mg
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx132084
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx132085
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx132086
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx174804
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  • 1 mg
  • 200 ug

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx238634-100ug 100 ug
EUR 661.2

Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx104947
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  • 100 ug
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  • 1 mg
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx116110
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  • 150 ul
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103325
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  • 100 ug
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  • 1 mg
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103326
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103328
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx103329
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  • 100 ug
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

20-abx001368
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Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx001368-100l 100 µl
EUR 400

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx001368-20l 20 µl
EUR 175

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx001368-50l 50 µl
EUR 275

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx116110-100g 100 µg Ask for price

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx116110-10g 10 µg
EUR 612.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx116110-200g 200 µg Ask for price

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx129517-100l 100 µl
EUR 262.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx129517-1ml 1 ml
EUR 687.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx129517-200l 200 µl
EUR 325

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx104947-100g 100 µg
EUR 475

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx104947-20g 20 µg
EUR 212.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx104947-50g 50 µg
EUR 250

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx132084-100tests 100 tests
EUR 237.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx132085-01mg 0.1 mg
EUR 275

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx132086-01mg 0.1 mg
EUR 237.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103325-100g 100 µg
EUR 475

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103325-20g 20 µg
EUR 212.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103325-50g 50 µg
EUR 250

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103326-100g 100 µg
EUR 475

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103326-20g 20 µg
EUR 212.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103326-50g 50 µg
EUR 250

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103328-100g 100 µg
EUR 725

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103328-20g 20 µg
EUR 262.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103328-50g 50 µg
EUR 337.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103329-100g 100 µg
EUR 725

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103329-20g 20 µg
EUR 262.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx103329-50g 50 µg
EUR 337.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx238634-100l 100 µl Ask for price

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx238634-50l 50 µl
EUR 350

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx174804-25mg 25 mg Ask for price

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx174804-5mg 5 mg
EUR 775

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx349963-96tests 96 tests
EUR 287.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx433382-100g 100 µg
EUR 387.5

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx323593-100g 100 µg
EUR 250

Tissue Factor Pathway Inhibitor (TFPI) Antibody

abx323593-50g 50 µg
EUR 187.5

SRE Reporter Kit (MAPK/ERK Signaling Pathway )

60511 500 rxns.
EUR 595
Description: The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.

SRE Reporter Kit (MAPK/ERK Signaling Pathway )

GWB-PS60EE 500reactions Ask for price

ISRE Reporter Kit (JAK/STAT Signaling Pathway)

60613 500 rxns.
EUR 565
Description: The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.

SBE Reporter Kit (TGFβ/SMAD signaling pathway)

60654 500 rxns.
EUR 595
Description: The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

FOXO Reporter Kit (PI3K/AKT Signaling Pathway)

GWB-PS252F 500reactions Ask for price

Eukaryotic Tissue Factor Pathway Inhibitor (TFPI)

EPA394Hu61 10ug
EUR 200

WNT1-inducible-signaling pathway protein 1

AP89136 1mg
EUR 2640

WNT1-inducible-signaling pathway protein 2

AP89180 1mg
EUR 2640

WNT1-inducible-signaling pathway protein 2

AP89190 1mg
EUR 2640

WNT1-inducible-signaling pathway protein 1

AP89218 1mg
EUR 2640

WNT1-inducible-signaling pathway protein 3

AP89505 1mg
EUR 2640

WNT1-inducible-signaling pathway protein 2

AP88795 1mg
EUR 2640

WNT1-inducible-signaling pathway protein 1

AP78813 1mg
EUR 2640

Eukaryotic Tissue Factor Pathway Inhibitor (TFPI)

RPU58805-100ug 100ug
EUR 672

Eukaryotic Tissue Factor Pathway Inhibitor (TFPI)

RPU58805-1mg 1mg
EUR 3120

Eukaryotic Tissue Factor Pathway Inhibitor (TFPI)

RPU58805-50ug 50ug
EUR 495

Anti-Tissue Factor Pathway Inhibitor antibody

STJ70019 100 µg
EUR 430.8

Rat Tissue factor pathway inhibitor ELISA kit

E02T0509-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Tissue factor pathway inhibitor ELISA kit

E02T0509-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Tissue factor pathway inhibitor ELISA kit

E02T0509-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Tissue factor pathway inhibitor ELISA kit

E08T0509-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Tissue factor pathway inhibitor ELISA kit

E08T0509-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Tissue factor pathway inhibitor ELISA kit

E08T0509-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Tissue factor pathway inhibitor ELISA kit

E07T0509-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Tissue factor pathway inhibitor ELISA kit

E07T0509-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Tissue factor pathway inhibitor ELISA kit

E07T0509-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Tissue factor pathway inhibitor ELISA kit

E01A16983 96T
EUR 700
Description: ELISA

ARE Report Cell Line (Nrf2 antioxidant pathway)

ABC-RC120D 1 vial Ask for price
Description: The ARE Reporter andndash; Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.

Rat Tissue Factor Pathway Inhibitor ELISA Kit

IRTTFPIKT each
EUR 718
Description: Rat Tissue Factor Pathway Inhibitor ELISA Kit

Tissue Factor Pathway Inhibitor 2 Rabbit pAb

E2121322 100ul
EUR 225
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44028-100ug 100ug
EUR 517

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44028-1mg 1mg
EUR 2293.2

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44028-50ug 50ug
EUR 415.8

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44029-100ug 100ug
EUR 443.3

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44029-1mg 1mg
EUR 1747.2

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44029-50ug 50ug
EUR 268.8

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44030-100ug 100ug
EUR 566.5

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44030-1mg 1mg
EUR 2511.6

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44030-50ug 50ug
EUR 455.4

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44031-100ug 100ug
EUR 517

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44031-1mg 1mg
EUR 2293.2

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44031-50ug 50ug
EUR 415.8

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44032-100ug 100ug
EUR 517

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44032-1mg 1mg
EUR 2293.2

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU44032-50ug 50ug
EUR 415.8

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU50613-100ug 100ug
EUR 431.2

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU50613-1mg 1mg
EUR 1911

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPU50613-50ug 50ug
EUR 346.5

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPA394Hu01 10ug
EUR 168

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

4-RPA394Hu01
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

RPA394Hu02 10ug
EUR 168

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

4-RPA394Hu02
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  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Tissue Factor Pathway Inhibitor expressed in: E.coli

We named this technique dual-terminal amplification (DTA) analysis. Results: By utilizing the DTA analysis, each in situ fluorescence imaging analysis and ex situ fluorescence quantitative analysis of MUC1 have been achieved. In addition, the aptamer-containing DNA ternary complicated stays on cell floor solely through the analysis and leaves the cell after the analysis is full. The cells could be maintained in a non-interfering state for the remaining of the time.

So after the analysis, it’s discovered that there aren’t any impact on the physiological exercise of cells and the expression of goal protein even after two rounds of repeatable imaging and quantitative analysis. Conclusion: In abstract, we’ve efficiently constructed a technique for nondestructive analysis of membrane protein in living cells.

We consider that this technique offers a promising approach for the analysis of the important thing membrane proteins of cells and the versatile utilization of treasured cell samples.

 

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