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Guidelines for Optimizing PCR: Concentrations of the Target DNA and Primers

Guidelines for Optimizing PCR: Introduction

Epigenetics Research Explores New Cancer Treatments

Gene Assembly: Extending Sequence Results by Primer Walking

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DNA Analysis, LLC

Your gateway to quality DNA sequencing services

and

fragment analysis services

.......

Long Read Lengths*Accurate Results*Fast Turn-around

DNA Analysis, LLC is a commercial provider of DNA sequencing services and fragment analysis services. We are committed to providing you with quality services and personal attention to your research needs. Our company founders spent several years in an academic core lab environment. Therefore the company was built with the understanding that establishing a lasting professional relationship with you, the researcher, is an important part of our business. Our experienced laboratory personnel are readily available to consult with directly by phone, or respond quickly to emails.

 

About Our DNA Sequencing Service

We have an extremely high success rate when you submit quality samples to be analyzed using our DNA sequencing services. Experienced laboratory personnel review each result and edit software-generated base miscalls. Completed results are uploaded to your free account on a secure FTP site for you to download after you receive our email notification. We have specialized chemistries designed to troubleshoot problematic templates that may contain hairpins or poly base regions, or have highly GC rich regions.  Your template and primer submissions are stored for 3 months for future use. We also bank primers beyond the 3 month storage period at your request with no additional cost.

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Quality Assessment of DNA Sequencing Templates

Commercial facilities sometimes offer free reloads when samples fail to produce sequence. However, if there is a problem with the sample or primer this does nothing to improve the result. We do a sample analysis using Nanodrop technology as well as other methods to provide you with a quality assessment of templates and primers. In most cases we are able to identify the problem related to sequencing. We then explain it to you and discuss various means of resolution. This also provides important feedback for future sample submissions. The first set of samples you submit for our DNA sequencing services will be quality assessed with the Nanodrop at no charge.

About Our Fragment Analysis Services

We provide full fragment analysis services and for products you amplified with fluorescent markers using capillary technology. The combination of capillary technology and fluorescent labeled markers will allow you to multiplex markers into a single sample. We use GS500 as our size standard for products ranging 100 bp to 450 bp. GS2500 is available for larger products. Samples submitted for fragment analysis services will be analyzed and reviewed by experienced laboratory personnel. We recommend that you contact us before submitting fragment analysis samples so we can evaluate the specific needs of your project and devise an optimal analysis strategy.

fragmentanalysis

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Click the link below to learn more

or contact us by phone at 513-841-2428 or email

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DNA Analysis, LLC
3921 Montgomery Road, Cincinnati, Ohio 45212
(P) 513.841.2428  (F) 513.531.2492

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